Background: In single cell DNA and RNA sequencing experiments, the number of cells to sequence must be\ndecided before running an experiment, and afterwards, it is necessary to decide whether sufficient cells were\nsampled. These questions can be addressed by calculating the probability of sampling at least a defined number of\ncells from each subpopulation (cell type or cancer clone).\nResults: We developed an interactive web application called SCOPIT (Single-Cell One-sided Probability Interactive\nTool), which calculates the required probabilities using a multinomial distribution (www.navinlab.com/SCOPIT). In\naddition, we created an R package called pmultinom for scripting these calculations.\nConclusions: Our tool for fast multinomial calculations provide a simple and intuitive procedure for prospectively\nplanning single-cell experiments or retrospectively evaluating if sufficient numbers of cells have been sequenced.\nThe web application can be accessed at navinlab.com/SCOPIT.
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